Overcoming challenges to imaging and manipulating
neuronal activity with light
Mammalian brains scatter light strongly
We image fluorophores that emit red light less scattered than shorter wavelengths
and develop post-processing methods to mitigate blur caused by scattering.
Neurons communicate with small, rapid signals
Resolving the smallest and fastest of these signals requires high photon fluxes to overcome shot noise. We develop high photon budget neuronal imaging methods based on widefield,
and light field microscopy.